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A novel open‐tubular capillary electrochromatography with magnetic nanoparticle coating as stationary phase
Author(s) -
Zhu Yaxian,
Zhou Chaoran,
Qin Sasa,
Ren Zhiyu,
Zhang Lingyi,
Fu Honggang,
Zhang Weibing
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100337
Subject(s) - capillary electrochromatography , magnetic nanoparticles , capillary action , nanoparticle , coating , chromatography , adsorption , materials science , elution , magnetic separation , aqueous solution , phase (matter) , electrochromatography , analytical chemistry (journal) , chemistry , nanotechnology , composite material , organic chemistry , metallurgy
A novel open‐tubular capillary electrochromatography (OT‐CEC) with modified core/shell magnetic nanoparticles coating as stationary phase was introduced using external magnetic force to fix magnetic nanoparticles. The magnetic nanoparticles coating inside the capillary columns could be easily regenerated by removing and re‐applying the external magnetic field. Magnetic field intensity, concentration and flow rate of nanoparticles suspension were investigated to achieve simple and stable preparation. Mixture of five organic acids was used as the marker sample to evaluate the OT‐CEC system, and the relative column efficiency of anthranilic acid reaches 220 000 plates/m. The excellent within‐column and between‐column repeatability has been testified with the RSDs of retention time of less than 1.51 and 5.29%, respectively. The aqueous extract of rhizoma gastrodiae was analyzed by the OT‐CEC system, and 23 peaks were eluted in 30 min. Compared with conventional open‐tubular capillary column, this new system shows faster separation speed and higher column efficiency from the larger surface area of nanoparticles. It has great potential in the method development for the analysis of complex samples, since magnetic coating can effectively prolong the column life by expediently replacing stationary phase to eliminate the pollution or irreversible adsorption.