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Recent advances in hydrophilic interaction liquid chromatography (HILIC) for structural glycomics
Author(s) -
Zauner Gerhild,
Deelder André M.,
Wuhrer Manfred
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100247
Subject(s) - hydrophilic interaction chromatography , chemistry , glycoproteomics , glycomics , chromatography , glycan , high performance liquid chromatography , biochemistry , glycoprotein
This review presents recent progress in employing hydrophilic interaction liquid chromatography (HILIC) for glycan and glycopeptides analysis. After an introduction of this technique, the following themes are addressed: (i) implementation of HILIC in large‐scale studies for analyzing the human plasma N ‐glycome; (ii) the use of HILIC UPLC (ultrahigh pressure liquid chromatography) for fast high‐resolution runs and its successful application with online MS for glycan and glycopeptide analysis; (iii) high‐throughput profiling using HILIC solid‐phase extraction in combination with MS detection; (iv) HILIC sample preparation for CE and CGE; (v) the latest glycoproteomic approaches implementing HILIC separation; (vi) future perspectives of HILIC including its use in large‐scale glycoproteomics studies such as the analysis of entire glycoproteomes at the glycopeptide level.