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High‐throughput functional marker assay for detection of Xa/xa and fgr genes in rice ( Oryza sativa L.)
Author(s) -
Salgotra Romesh K.,
Millwood Reginald J.,
Agarwal Sujata,
Stewart C. Neal
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100196
Subject(s) - oryza sativa , biology , gene , genetics , allele , marker assisted selection , multiplex polymerase chain reaction , genotype , bacterial blight , microbiology and biotechnology , polymerase chain reaction
We apply CE for high‐throughput analysis of functional markers for marker‐assisted selection in rice. The accuracy, throughput and reproducibility of CE analysis for sequence‐tagged site (STS) and simple sequence repeat (SSR) markers for bacterial blight resistance and aroma genes are demonstrated by using a CE system. Multiplex PCR products displayed well‐differentiated allelic variants using different STS and SSR markers for identification of xa13 , Xa21 and fgr genes using the CE system compared to 1.2% agarose gel images. Moreover, consumption of PCR product is much less in the CE system compared to traditional agarose gel systems. Sample consumption is less than 0.1 μL per analysis, thereby conserving samples for further downstream analysis. Out of 29 genotypes in BC 1 F 3 generation, 16 plants were found homozygous for all the three genes, viz., xa13 , Xa21 and fgr . These homozygous lines can be used as potential donors in rice breeding programmes.