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Ionic liquid‐assisted SDS‐PAGE to improve human serum protein separation
Author(s) -
Zhang Tao,
Gai Qingqing,
Qu Feng,
Zhang Yukui
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100184
Subject(s) - chromatography , polyacrylamide gel electrophoresis , chemistry , lysozyme , bovine serum albumin , gel electrophoresis , sodium dodecyl sulfate , sodium , biochemistry , organic chemistry , enzyme
Ionic liquid (IL)‐assisted sodium dodecyl sulfate polyacrylamide gel electrophoresis (ILs‐SDS‐PAGE) was presented to improve protein separation. ILs were employed during the preparation process of polyacrylamide gel, then the modified gel was used for commercial protein marker, binary bovine serum albumin/lysozyme (BSA/Lyz) and human serum separation. The influence of ionic liquid concentration, cation alkyl chain length, cation and anion types on proteins separation were investigated. The results showed that ILs played a role in improving some protein separation, and ILs‐SDS‐PAGE provided higher resolution and separation efficiency than ordinary SDS‐PAGE for low and middle relative molecular mass proteins in human serum. In addition, the principle of ILs‐SDS‐PAGE was discussed and the comparison of ILs‐SDS‐PAGE with ordinary SDS‐PAGE and Native PAGE was made.