Premium
Trapping and imaging of micron‐sized embryos using dielectrophoresis
Author(s) -
Khoshmanesh Khashayar,
Kiss Nimrod,
Nahavandi Saeid,
Evans Clive W.,
Cooper Jonathan M.,
Williams David E.,
Wlodkowic Donald
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100160
Subject(s) - dielectrophoresis , trapping , optical tweezers , materials science , nanotechnology , optics , biology , physics , microfluidics , ecology
Development of dielectrophoretic (DEP) arrays for real‐time imaging of embryonic organisms is described. Microelectrode arrays were used for trapping both embryonated eggs and larval stages of Antarctic nematode Panagrolaimus davidi . Ellipsoid single‐shell model was also applied to study the interactions between DEP fields and developing multicellular organisms. This work provides proof‐of‐concept application of chip‐based technologies for the analysis of individual embryos trapped under DEP force.