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Size separation of proteins by capillary zone electrophoresis with cationic hitchhiking
Author(s) -
Dolnik Vladislav,
Gurske William A.
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100114
Subject(s) - capillary electrophoresis , repeatability , chromatography , cationic polymerization , electrokinetic phenomena , electrophoresis , chemistry , pulmonary surfactant , capillary action , detection limit , analytical chemistry (journal) , separation method , materials science , biochemistry , organic chemistry , composite material
The paper describes a method of size separation of proteins by capillary sieving electrophoresis with cationic surfactant. Proteins are separated within 12 min with repeatability of migration times better than 0.2%. Some proteins achieve the separation efficiency of 200 000 theoretical plates. The method can be used for determination of protein relative molecular masses. The accuracy of the determined relative molecular masses and the limitation of the method were investigated by the analysis of more than 60 proteins. The method also allows separation of protein oligomers. Proteins can be quantitated after the electrokinetic injection in the concentration range 0.07–0.43 g/L. The average detection limit is about 2 mg/L.