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Rapid, automated measurement of dielectrophoretic forces using DEP‐activated microwells
Author(s) -
Broche Lionel M.,
Hoettges Kai F.,
Ogin Stephen L.,
Kass George E. N.,
Hughes Michael P.
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100063
Subject(s) - dielectrophoresis , electric field , biological system , electrode , noise (video) , population , particle (ecology) , nanotechnology , materials science , chemistry , optoelectronics , computer science , physics , artificial intelligence , biology , microfluidics , ecology , demography , quantum mechanics , sociology , image (mathematics)
Abstract Dielectrophoresis (DEP) is a physical effect that generates a force on polarisable particles experiencing a non‐homogeneous electric field; studying the effect as a function of frequency allows the determination of the electrical properties of that particle, i.e. the electrical permittivity and conductivity. In the past, DEP‐based techniques applied to the measurement of one or several cells at a time have been subject to many sources of noise, which result in an ambiguous or inaccurate result. However, improvements are possible by generating more information from the experiments. In this paper, we present a rapid automated system that measures the DEP spectrum from a large population of cells with a low level of noise using the microwell electrodes, based on a method of analysis that provides additional information about the electrical properties of the cells and a new theoretical approach was developed to obtain accurate, bias‐free results in <5 min.