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Large‐volume sample on‐line concentration of angiotensins in non‐aqueous capillary electrophoresis
Author(s) -
Kitagawa Shinya,
Noji Akitsugu,
Matsushita Naofumi,
Nakagawa Hiroyuki,
Asai Takahiro,
Ohitani Hajime
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000673
Subject(s) - chemistry , capillary electrophoresis , chromatography , electrophoresis , capillary action , perchlorate , trifluoroacetic acid , aqueous solution , tetraethylammonium , volume (thermodynamics) , analyte , analytical chemistry (journal) , materials science , potassium , ion , organic chemistry , quantum mechanics , composite material , physics
A single step on‐line concentration and separation method for peptides in non‐aqueous capillary electrophoresis was developed. ACN containing 50 mM tetraethylammonium perchlorate was used as the electrophoretic medium; angiotensins I–IV were separated as a result of the differences in the magnitudes of their interactions with perchlorate anions. When the sample solution (ACN containing 0.5% trifluoroacetic acid and angiotensins) was injected as a large‐volume plug, the analytes were concentrated at the inlet end of the capillary by both sweeping and stacking mechanisms; the separation procedure then started automatically without any operations such as polarity change. It was found that the concentration of analytes, injection period, and concentration of tetraethylammonium perchlorate in the electrophoretic medium were important factors for both separation and concentration efficiencies. The angiotensins were concentrated and separated with the large‐volume injection of up to 80% of the effective capillary length.