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Nanoband electrode for high‐performance in‐channel amperometric detection in dual‐channel microchip capillary electrophoresis
Author(s) -
Chen Chuanpin,
Teng Wei,
Hahn Jong Hoon
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000661
Subject(s) - amperometry , capillary electrophoresis , polydimethylsiloxane , detection limit , detector , electrode , materials science , analyte , analytical chemistry (journal) , resolution (logic) , capillary action , chromatography , optoelectronics , chemistry , electrochemistry , optics , nanotechnology , physics , composite material , artificial intelligence , computer science
A nanoband electrode detector integrated with a dual‐channel polydimethylsiloxane microchip is proposed for in‐channel amperometric detection in microchip capillary electrophoresis. Gold nanoband electrodes, which were fabricated on SU‐8 substrates with a 100‐nm‐width gold layer, were introduced into the dual‐channel microchip to be an electrochemical detector. Due to the nano‐sized width of the detector, the noise of the amperometric detection was significantly reduced, and a high separation resolution was achieved for monitoring the analytes. The detection sensitivity of the system was improved by high signal‐to‐noise ratio, and a low detection limit on microchip was obtained for p ‐aminophenol (2.09 nM). Because of the high resolution in measuring half‐peak width, the plate number that is used to evaluate the separation efficiency was 1.5‐fold higher than that using 50‐μm‐width electrochemical detector. The effect of sample injection time and data acquisition time on separation efficiency was investigated, and an attractive separation efficiency was achieved with a plate number up to 17 500.