Premium
Capillary electrophoresis immunoassays with conjugated quantum dots
Author(s) -
Klepárník Karel,
Voráčová Ivona,
Lišková Marcela,
Přikryl Jan,
Hezinová Věra,
Foret František
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000652
Subject(s) - quantum dot , quantum yield , capillary electrophoresis , analytical chemistry (journal) , fluorescence , luminescence , conjugated system , materials science , conjugate , buffer solution , emission spectrum , immunoassay , chemistry , chromatography , optoelectronics , spectral line , optics , antibody , polymer , mathematical analysis , physics , mathematics , astronomy , immunology , composite material , biology
Water‐soluble CdTe quantum dots (QDs) and their conjugates with antibodies and antigenes were prepared by optimized procedures for applications in CE immunoassays. The QD size of 3.5 nm, excitation spectrum in the range of 300–500 nm, the maximum wavelength of the emission spectrum at 610 nm, quantum yield of 0.25 and luminescence lifetimes in the range of 3.6–43 ns were determined. The 0.1 M solution of TRIS/TAPS (pH 8.3) was found to be the optimum buffer for the separation of the antiovalbumin–ovalbumin immunocomplex from the free conjugates of QDs.