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Solid‐phase extraction‐field‐amplified sample injection coupled with CE‐ESI‐MS for online pre‐concentration and quantitative analysis of brain‐gut peptides
Author(s) -
Ye Hongzhi,
Xia Shifei,
Yu Lishuang,
Xu Xueqin,
Zheng Chunsong,
Xu Huifeng,
Wang Lili,
Liu Xianxiang,
Cai Zongwei,
Chen Guonan
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000591
Subject(s) - chromatography , solid phase extraction , extraction (chemistry) , stacking , chemistry , cerebrospinal fluid , sample preparation , quantitative analysis (chemistry) , matrix (chemical analysis) , peptide , analytical chemistry (journal) , medicine , biochemistry , organic chemistry
In order to improve the sensitivity of CE‐ESI‐MS for the analysis of brain‐gut peptides, a solid‐phase extraction combined with field‐amplified sample injection was used for the pre‐concentration of the brain‐gut peptides. Compared with the conventional pressure injection method, the sensitivity in the detection of brain‐gut peptides was improved more than 100‐fold. The possible factors affecting sample stacking, such as the sample matrix, the composition and the length of the water column, the types and the volumes of eluent, have been investigated in detail. Under the optimum conditions, the detectable concentration of brain‐gut peptides was found to be as low as 0.02 μM. A linear response concentration for the detection was developed in the range of 0.08–25 μmol/L. A real sample of human cerebrospinal fluids, which was spiked with brain‐gut peptides, was also examined in order to evaluate the reliability of the proposed approach. The recovery of the method was in a range from 69.2 to 85.4%. The method was found to be reliable, accurate and potentially applicable for clinical drug analysis.