Premium
Evaluation of the free radical scavenging capability of wheat extracts by capillary electrophoresis and multivariate curve resolution
Author(s) -
Helmja Kati,
Vaher Merike,
Kaljurand Mihkel
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000576
Subject(s) - scavenging , chemistry , capillary electrophoresis , resolution (logic) , chromatography , bran , syringic acid , rutin , ferulic acid , electropherogram , radical , organic chemistry , gallic acid , antioxidant , raw material , artificial intelligence , computer science
In the current work, phenolic acids were analysed and their radical scavenging activity evaluated by CE, including the quantification of overlapping peaks by applying the multivariate curve resolution (MCR) method. To investigate radical scavenging activity, the solution of phenolic acids was treated with a stable free 2,2‐diphenyl‐1‐picrylhydrazyl radical of different concentrations and the radical scavenging activity of the reaction mixture was monitored. The radical scavenging reaction was also applied to wheat bran extracts. The overlapping peaks on the electropherograms of both mixtures of standard compounds and extracts of different wheat bran varieties were resolved by using the MCR method, assuming that the overlapping compounds have different UV spectra. MCR enabled the insufficient resolution between syringic and ferulic acids to be effectively resolved.