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Mass spectrometry detection as an innovative and advantageous tool in ligand exchange capillary electrophoresis
Author(s) -
Giuffrida Alessandro,
Contino Annalinda,
Maccarrone Giuseppe,
Messina Marianna,
Cucinotta Vincenzo
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000570
Subject(s) - capillary electrophoresis , chemistry , mass spectrometry , chromatography , electrospray , detection limit , resolution (logic) , electrospray mass spectrometry , cyclodextrin , capillary electrophoresis–mass spectrometry , ligand (biochemistry) , analytical chemistry (journal) , electrophoresis , enantiomer , electrospray ionization , stereochemistry , biochemistry , receptor , artificial intelligence , computer science
The copper(II) complex of a modified cyclodextrin, namely 6‐mono‐deoxy‐6‐[4‐(2‐aminoethyl)imidazolyl]‐β‐CD (CDmh), previously synthesized and characterized in our laboratory and already used as chiral selector for ligand exchange capillary electrophoresis (LECE) with optical detection, is investigated here in LECE using electrospray‐mass spectrometry (ESI‐MS) as the detection device. The potential of this hyphenated method is compared with the results previously obtained with optical detection by studying the chiral resolution of tryptophan racemate. Chiral separation conditions compatible with LECE‐ESI‐MS could be achieved based on the figures of merit obtained by LECE‐UV. Interestingly, the values of LOD obtained by LECE‐ESI‐MS were significantly better than those obtained by LECE‐UV and thus, ESI‐MS detection seems to open new perspectives in chiral separations by LECE.