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Hydroxyethylcellulose‐ graft ‐poly(2‐(dimethylamino)ethyl methacrylate) as physically adsorbed coating for protein separation by CE
Author(s) -
Cao Fuhu,
Luo Zhaofeng,
Zhou Dan,
Zeng Rongju,
Wang Yanmei
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000528
Subject(s) - methacrylate , copolymer , capillary electrophoresis , grafting , ceric ammonium nitrate , coating , polymer chemistry , capillary action , protein adsorption , adsorption , chemistry , materials science , chemical engineering , chromatography , polymer , organic chemistry , composite material , engineering
In this work, a novel graft copolymer, hydroxyethylcellulose‐ graft ‐poly(2‐(dimethylamino)ethyl methacrylate) (HEC‐ g ‐PDMAEMA), used as physical coatings of the bare fused‐silica capillaries, was synthesized by using ceric ammonium nitrate initiator in aqueous nitric acid solution. EOF measurement results showed that the synthesized HEC‐ g ‐PDMAEMA graft copolymer‐coated capillary in this paper could suppress EOF effectively compared to the bare fused‐silica capillary, and efficient separations of basic proteins were also achieved. The electrical charge of the coated capillary wall could be modulated by varying not only the pH of the running buffer, but also the grafting ratio of poly(2‐(dimethylamino)ethyl methacrylate) grafts, which makes possible the analysis of basic and acidic proteins in the same capillary. The effects of poly(2‐(dimethylamino)ethyl methacrylate) grafting ratio in HEC‐ g ‐PDMAEMA and buffer pH on the separation of basic proteins for capillary electrophoresis were investigated in detail. Furthermore, egg white proteins and milk powder samples were separated by the HEC‐ g ‐PDMAEMA‐coated capillary. The results demonstrated that the HEC‐ g ‐PDMAEMA copolymer coatings have great potential in the field of diagnosis and proteomics.