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Analysis of methylene blue and its metabolites in blood by capillary electrophoresis/electrospray ionization mass spectrometry
Author(s) -
Yang Fang,
Xia Shifei,
Liu Zhencai,
Chen Jian,
Lin Yonghui,
Qiu Bin,
Chen Guonan
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000514
Subject(s) - chromatography , electrospray ionization , chemistry , mass spectrometry , extraction (chemistry) , capillary electrophoresis , electrospray , methylene blue , electrospray mass spectrometry , biochemistry , photocatalysis , catalysis
Abstract A method for the determination of methylene blue (MB) and its metabolites (azure A, azure B and azure C) in rat blood by CE‐electrospray ionization mass spectrometry (CE‐ESI‐MS) was developed in this paper. Different analytical parameters were investigated in detail such as pH and concentration of separation buffer, and ESI‐MS instrumental parameters. Under the optimum conditions, MB and its metabolites were separated and detected in 27.3 min. LODs (defined as S / N =3) of this method were 0.22, 0.25, 0.10 and 0.30 μg/mL for MB, azure A, azure B and azure C, respectively. To get a satisfactory extraction efficiency of MB and its metabolites in rat blood, different extraction solutions were studied. By using this method, MB and its metabolites (azure A, azure B and azure C) were successfully analyzed in rat blood samples.