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Mucin (MUC5AC) expression by lung epithelial cells cultured in a microfluidic gradient device
Author(s) -
Kim Su Hwan,
Kang Jin Hyun,
Chung Il Yup,
Chung Bong Geun
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000501
Subject(s) - mucin , microfluidics , concentration gradient , lung , microbiology and biotechnology , cell culture , chemistry , biology , biophysics , materials science , biochemistry , nanotechnology , chromatography , medicine , genetics
We have developed a microfluidic gradient device for controlling mucin gene expression of NCI‐H292 epithelial cells derived from lung tissues. We hypothesized that gradient profiles would control mucin gene expression of lung epithelial cells. However, it was not possible to generate various stable gradient profiles using conventional culture methods. To address this limitation, we used a microfluidic gradient device to create various gradient profiles ( i.e. non‐linear, linear, and flat) in a temporal and spatial manner. NCI‐H292 lung epithelial cells were exposed to concentration gradients of epidermal growth factor in a microfluidic gradient device with continuous medium perfusion. We demonstrated an effect of gradient profiles on mucin expression of lung epithelial cells cultured in the microfluidic gradient device. It was revealed that NCI‐H292 lung epithelial cells exposed to the flat gradient profile of the epidermal growth factor exhibited high expression of mucin as compared with cells exposed to non‐linear and linear gradient profiles. Therefore, this microfluidic gradient device could be a potentially useful tool for regulating the mucin expression of lung epithelial cells exposed to chemokine gradient profiles.