Premium
Cationic poly(amidoamine) dendrimers as additives for capillary electroseparation and detection of proteins
Author(s) -
Liu Qingchun,
Tian Jing,
Zhang Chenling,
Yang Huan,
Liu Yang,
Qin Weidong,
Liu Zhengping
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000440
Subject(s) - poly(amidoamine) , dendrimer , chemistry , absorbance , cationic polymerization , amidoamine , chromatography , polymer chemistry
We examine the influence of cationic poly(amidoamine) (PAMAM) dendrimers on capillary electroseparation–UV analysis of proteins. PAMAMs adsorbing to the capillary surface suppressed the wall‐adsorption of proteins; meanwhile, PAMAMs added to the buffer exhibited selectivity toward proteins. Presence of 3×10 −4 g/mL PAMAM generation one (G 1.0) in 30 mM phosphate, at pH 2.6, rendered significant enhancement in separation efficiency; the merged peaks of myoglobin and trypsin inhibitor were separated. Moreover, the protein–dendrimer interactions changed the inherent UV absorbance profiles of proteins. UV–Vis study showed that the absorbance of cytochrome C and transferrin increased at the detection wavelength of 214 nm; their detection sensitivity enhanced by 2.44 and 2.01‐folds, respectively, with addition of 5×10 −4 g/mL PAMAM G 1.0.