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A SNaPshot assay for genotyping 44 individual identification single nucleotide polymorphisms
Author(s) -
Lou Chunguang,
Cong Bin,
Li Shujin,
Fu Lihong,
Zhang Xiaojing,
Feng Ting,
Su Shanshan,
Ma Chunling,
Yu Feng,
Ye Jian,
Pei Li
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000426
Subject(s) - genotyping , biology , multiplex , genetics , single nucleotide polymorphism , typing , amplicon , multiplex polymerase chain reaction , snp genotyping , microsatellite , snp , loss of heterozygosity , forensic identification , population , genotype , computational biology , polymerase chain reaction , allele , gene , medicine , environmental health
Abstract Single nucleotide polymorphisms (SNPs), which have relatively low mutation rates and can be genotyped after PCR with shorter amplicons compared with short tandem repeats (STRs), are being considered as potentially useful markers in forensic DNA analysis. Those SNPs with high heterozygosity and low Fst ( F ‐statistics) in human populations are described as individual identification SNPs, which perform the same function as STRs used in forensic routine work. In the present study, we developed a multiplex typing method for analyzing 44 selected individual identification SNPs simultaneously by using multiplex PCR reaction in association with fluorescent labeled single base extension (SBE) technique. PCR primers were designed and the lengths of the amplicons ranged from 69 to 125 bp. The population genetics data of 79 unrelated Chinese individuals for the 44 SNP loci were investigated and a series of experiments were performed to validate the characteristic of the SNP multiplex typing assay, such as sensitivity, species specificity and the performance in paternity testing and analysis of highly degraded samples. The results showed that the 44‐SNPs multiplex typing assay could be applied in forensic routine work and provide supplementary data when STRs analysis was partial or failed.

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