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Quantification of plasma lactate concentrations using capillary electrophoresis with contactless conductivity detection
Author(s) -
Pormsila Worapan,
Morand Réjane,
Krähenbühl Stephan,
Hauser Peter C.
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000420
Subject(s) - capillary electrophoresis , chromatography , chemistry , plasma , capillary action , electrophoresis , analytical chemistry (journal) , conductivity , materials science , composite material , physics , quantum mechanics
The quantification of plasma lactate and evaluation of the lactate threshold by CE with capacitively coupled contactless conductivity is demonstrated. The only sample preparation needed was deproteinization with a ACN/methanol mixture. A solution of 10 mmol/L 2‐morpholinoethanesulfonic acid monohydrate, 10 mmol/L DL ‐histidine, 70 μmol/L hexadecyltrimethylammonium bromide, pH 6.0 was found suitable as running buffer. Linearity was achieved for the concentration range of 10–1000 μmol/L with a correlation coefficient of 0.9994. The limit of detection (3 S/N) was determined as 3.2 μmol/L. Intra‐ and inter‐day variabilities were less than 7% RSD. The suitability of the method could be demonstrated by analyzing various clinical samples, where the results correlated satisfactorily with those of an established enzymatic method.