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Nanochitosan crosslinked with polyacrylamide as the chiral stationary phase for open‐tubular capillary electrochromatography
Author(s) -
Chen JianLian,
Hsieh KaiHsin
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000410
Subject(s) - capillary electrochromatography , stationary phase , polyacrylamide , chromatography , phase (matter) , chiral stationary phase , capillary action , chemistry , capillary electrophoresis , chemical engineering , materials science , polymer chemistry , high performance liquid chromatography , organic chemistry , composite material , engineering
Abstract Nanoparticles exhibiting favorable surface‐to‐volume ratios create efficient stationary phases for electrochromatography. New nanomaterials derived from chitosan (CS) were immobilized onto modified capillaries for use as the chiral stationary phase (CSP) in open‐tubular electrochromatography. This immobilization was achieved through the copolymerization of glycidyl methacrylate‐modified nano‐CS with methacrylamide (MAA) and bis‐acrylamide crosslinkers (forming the MAA‐CS capillary) rather than the attachment of nano‐CS to the copolymer of glycidyl methacrylate, MAA, and bis‐acrylamide (forming the MAA+CS capillary). The completed MAA‐CS capillary and its precursors were examined by SEM and ATR‐IR measurements. Before separating chiral samples, the MAA‐CS capillary was characterized by electroosmotic flow measurements at varying pH values, concentrations, and volume percentages of organic modifiers in the running buffers. Tryptophan enantiomers were well separated by the MAA‐CS capillary, whereas no enantioselectivity was observed in the MAA+CS capillary. With the addition of 80% MeOH into the phosphate buffer, the chiral separation of (±)‐catechin was accomplished in a normal‐phase mode. However, the new CSP has its limitations, as only two groups of α‐tocopherol stereoisomers were separated.