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Capillary electrophoresis–mass spectrometry for the analysis of intact proteins 2007–2010
Author(s) -
Haselberg Rob,
de Jong Gerhardus J.,
Somsen Govert W.
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000364
Subject(s) - chemistry , mass spectrometry , capillary electrophoresis , interfacing , chromatography , capillary electrophoresis–mass spectrometry , characterization (materials science) , analytical chemistry (journal) , electrospray ionization , nanotechnology , materials science , computer science , computer hardware
CE coupled to MS has proven to be a powerful analytical tool for the characterization of intact proteins, as it combines the high separation efficiency of CE with the selectivity of MS. This review provides an overview of the development and application of CE‐MS methods within the field of intact protein analysis as published between January 2007 and June 2010. Ongoing technological developments with respect to CE‐MS interfacing, capillary coatings for CE‐MS, coupling of CIEF with MS and chip‐based CE‐MS are treated. Furthermore, CE‐MS of intact proteins involving ESI, MALDI and ICP ionization is outlined and overviews of the use of the various CE‐MS methods are provided by tables. Representative examples illustrate the applicability of CE‐MS for the characterization of proteins, including glycoproteins, biopharmaceuticals, protein–ligand complexes, biomarkers and dietary proteins. It is concluded that CE‐MS is a valuable technique with high potential for intact protein analysis, providing useful information on protein identity and purity, including modifications and degradation products.