Premium
Sensitized phosphorescence as detection method for the enantioseparation of bupropion by capillary electrophoresis
Author(s) -
CastroPuyana María,
Lammers Ivonne,
Buijs Joost,
Gooijer Cees,
Ariese Freek
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000352
Subject(s) - phosphorescence , chromatography , chemistry , enantiomer , analyte , capillary electrophoresis , detection limit , bupropion , electrokinetic phenomena , micellar electrokinetic chromatography , analytical chemistry (journal) , organic chemistry , fluorescence , medicine , physics , quantum mechanics , pathology , smoking cessation
A new CE detection method was developed for the chiral drug bupropion (a second‐generation antidepressant), based on phosphorescence both in the direct and in the sensitized mode using pulsed laser excitation at 266 nm. Electrokinetic chromatography using 5 mM sulfated‐α‐CD as chiral selector in 25 mM phosphate buffer at pH 3 allowed the separation of bupropion enantiomers with a high chiral resolution (Rs>3). In the sensitized phosphorescence detection mode, excitation energy is transferred from the analyte to an acceptor (1‐bromo‐4‐napthhalenesulfonic acid or biacetyl) followed by time‐resolved phosphorescence detection under deoxygenated buffer conditions. Using 2×10 −4 M biacetyl as the acceptor an LOD of 2×10 −7 M was obtained for each enantiomer, about 40 times better than in the direct mode. Under these separation conditions, no significantly different phosphorescence lifetimes (measured on‐line) were obtained for the two bupropion enantiomers. The suitability of the method was demonstrated with the quantification of bupropion in a pharmaceutical formulation and its determination in a spiked urine sample.