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Enantioseparation of DL ‐isocitric acid by a chiral ligand exchange CE with Ni(II)‐ D ‐quinic acid system
Author(s) -
Kodama Shuji,
Taga Atsushi,
Yamamoto Atsushi,
Ito Yuji,
Honda Yoshitaka,
Suzuki Kentaro,
Yamashita Tomohisa,
Kemmei Tomoko,
Aizawa Senichi
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000320
Subject(s) - chemistry , citric acid , ligand (biochemistry) , quinic acid , enantiomer , acetic acid , metal , ion exchange , metal ions in aqueous solution , ion , nuclear chemistry , stereochemistry , organic chemistry , biochemistry , receptor
The ratio of citric acid to D ‐isocitric acid can be used to distinguish authentic and adulterated fruit juices. To separate DL ‐isocitric acid enantiomers, we used ligand exchange CE. D ‐Quinic acid was used as a chiral selector ligand and Mn(II), Fe(III), Co(II), Ni(II), Cu(II), and Zn(II) ions were used as the central ions of the chiral selector in the BGE. DL ‐Isocitric acid was found to be enantioseparated with the above metal ions except for Mn(II) ion. The optimum running conditions for the analysis of D ‐ and L ‐isocitric acids along with citric acid, an isomer of isocitric acid, were found to be a BGE (pH 5.0) containing 30% ACN, 20 mM acetic acid, 20 mM NiSO 4 , and 80 mM D ‐quinic acid. Under these conditions, DL ‐isocitric and citric acids in fruit juices were analyzed successfully.