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Highly sensitive trivalent copper chelate‐H 2 O 2 system for CE‐chemiluminescent detection of luminol‐type compounds
Author(s) -
Fu Zhifeng,
Li Zongyun,
Xie Haoyue,
Li Tao,
Li Cuifang
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000310
Subject(s) - luminol , chemistry , chemiluminescence , derivatization , copper , chelation , reagent , periodate , detection limit , biomolecule , inorganic chemistry , chromatography , organic chemistry , high performance liquid chromatography , biochemistry
Luminol‐type compounds can be used as chemiluminescent (CL) derivatization reagents for amines, carboxylic acids and protein. Copper chelate diperiodatocuprate(III) (K 5 [Cu(HIO 6 ) 2 ], DPC) was synthesized by complexation of copper at trivalent oxidation state and periodate in a strong basic medium. It was found that DPC can greatly enhance the reaction between luminol‐type compounds and H 2 O 2 to produce very strong CL emission. Based on this fact, a rapid CE method combined with high‐sensitive end‐column CL detection was established to simultaneously analyze luminol and N ‐(4‐aminobutyl)‐ N ‐ethylisoluminol (ABEI) with wide concentration range of 3.0–300 nmol/L in 5 min. The RSDs of the signal intensity and the migration time were less than 3.9 and 7.0% for a standard sample containing 100 nmol/L luminol and ABEI ( n =5), respectively. The investigation implies that DPC is a promising sensitizer for CE‐CL detection of a great variety of biomolecules and drugs in biological samples after derivatization using luminol derivatives.