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Application of microchip CGE for the analysis of PEG‐modified recombinant human granulocyte‐colony stimulating factors
Author(s) -
Park Eun Ji,
Lee Kyung Soo,
Lee Kang Choon,
Na Dong Hee
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000302
Subject(s) - pegylation , peg ratio , granulocyte colony stimulating factor , recombinant dna , chromatography , chemistry , granulocyte , polyethylene glycol , biochemistry , biology , medicine , immunology , finance , chemotherapy , economics , gene
The purpose of this study was to evaluate the microchip CGE (MCGE) for the analysis of PEG‐modified granulocyte‐colony stimulating factor (PEG‐G‐CSF) prepared with PEG‐aldehydes. The unmodified and PEG‐modified G‐CSFs were analyzed by Protein 80 and 230 Labchips on the Agilent 2100 Bioanalyzer. The MCGE allowed size‐based separation and quantitation of PEG‐G‐CSF. The Protein 80 Labchip was useful for PEG‐5K‐G‐CSF, while the Protein 230 Labchip was more suitable for PEG‐20K‐G‐CSF. The MCGE was also used to monitor a search for optimal PEG‐modification (PEGylation) conditions to produce mono‐PEG‐G‐CSF. This study demonstrates the usefulness of MCGE for monitoring and optimizing the PEGylation of G‐CSF with the advantages of speed, minimal sample consumption, and automatic quantitation.