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Study on the influence of cross‐sectional area and zeta potential on separation for hybrid‐chip‐based capillary electrophoresis using 3‐D simulations
Author(s) -
Wu ZengQiang,
Cao XiaoDan,
Chen Lu,
Zhang JianRong,
Xia XingHua,
Fang Qun,
Chen HongYuan
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000258
Subject(s) - chip , materials science , capillary electrophoresis , capillary action , channel (broadcasting) , analytical chemistry (journal) , optoelectronics , chemistry , chromatography , computer science , telecommunications , composite material
Hybrid chips combing microchips with capillaries have displayed particular advantages in achieving UV‐vis and mass spectroscopic detection. In this work, systematic 3‐D numerical simulations have been carried out to explore the influence of junction interface cross‐sectional area and ζ‐potential distribution on sample band broadening in hybrid‐chip electrophoresis separation. In this case, the ratio of cross‐sectional area of chip to capillary channel ( S ratio ) is used as the parameter of the variation in junction interface cross‐sectional area. Theoretical simulations demonstrated that the decrease of the S ratio would increase the separation efficiency in the hybrid‐chip‐based CE with uniform ζ‐potential distribution. ζ‐potential distribution along the axial direction of the channel also affects mass transport in hybrid‐chip‐based CE. Therefore, the effect of ζ‐potential distribution has been considered in the 3‐D simulation. Theoretical simulation results reveal that ζ‐potential distribution rather than the interface cross‐sectional area variation ( S ratio ) controls the sample band broadening and manipulates sample separation efficiency in the hybrid‐chip‐based CE with non‐uniform ζ‐potential distribution. Both the theoretical simulations and experimental results show that optimal hybrid‐chip CE separation efficiency can be achieved at S ratio =1.