Premium
Comparative enantioseparations of pharmaceuticals in capillary electrochromatography on polysaccharide‐based chiral stationary phases containing selectors with or without chlorinated derivatives
Author(s) -
Hendrickx Ans,
Mangelings Debby,
Chankvetadze Bezhan,
Vander Heyden Yvan
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000249
Subject(s) - chemistry , chlorine , cellulose , capillary electrochromatography , tris , chromatography , chiral stationary phase , polysaccharide , combinatorial chemistry , organic chemistry , capillary electrophoresis , high performance liquid chromatography , biochemistry
The screening conditions of an existing chiral strategy in CEC were tested for their applicability on four chlorine‐containing polysaccharide‐based stationary phases. The selectors of these phases are cellulose tris (3‐chloro‐4‐methylphenylcarbamate), amylose tris (5‐chloro‐2‐methylphenylcarbamate), cellulose tris (4‐chloro‐3‐methylphenylcarbamate) and cellulose tris (3,5‐dichlorophenylcarbamate). The enantioselectivity of these phases was compared with those of the four phases without chlorine (Chiralpak ® AD‐RH, Chiralcel ® OD‐RH, Chiralpak ® AS‐RH and Chiralcel ® OJ‐RH) used in the earlier defined strategy. A test set of 48 structurally diverse drug compounds was analyzed using the screening conditions of the strategy. These results led to possibilities to upgrade the current screening strategy so that improved success rates are obtained. The chlorine‐containing chiral stationary phases demonstrated an added value to the screening process since they showed enantioresolution for compounds not resolved by the chiral stationary phases not containing chlorine in their structure.