Premium
Polymeric nanopillar arrays for cell traction force measurements
Author(s) -
Kuo ChiungWen,
Shiu JauYe,
Chien FanChing,
Tsai ShihMin,
Chueh DiYan,
Chen Peilin
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000212
Subject(s) - nanopillar , materials science , nanotechnology , etching (microfabrication) , traction (geology) , optoelectronics , nanostructure , mechanical engineering , layer (electronics) , engineering
This paper reports the development of a novel force measurement device based on polymeric nanopillar arrays. The device was fabricated by a process combining nanosphere lithography, oxygen plasma treatment, deep etching and nano‐molding. Well‐ordered polymeric nanopillar arrays with various diameters and aspect ratios were fabricated and used as cell culture substrates. Cell traction forces were measured by the deflection of the nanopillars. Since the location of the nanopillars can be monitored at all times, this device allows for the measurement of the evolution of adhesion forces over time.