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Polymer monolith‐integrated multilayer poly(dimethylsiloxane) microchip for online microextraction and capillary electrophoresis
Author(s) -
Kang QinShu,
Li Yun,
Xu JunQing,
Su LiJin,
Li YuTao,
Huang WeiHua
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000210
Subject(s) - monolith , materials science , polymer , polymerization , capillary electrophoresis , photopolymer , molecularly imprinted polymer , monolithic hplc column , ethylene glycol dimethacrylate , elution , microchannel , chemical engineering , chromatography , capillary action , methacrylic acid , nanotechnology , chemistry , composite material , selectivity , biochemistry , engineering , catalysis
We reported the in situ synthesis and use of porous polymer monolith (PPM) columns in an integrated multilayer PDMS/glass microchip for microvalve‐assisted on‐line microextraction and microchip electrophoresis for the first time. Under the control of PDMS microvalves, the grafting of the microchannel surface and in situ photopolymerization of poly(methacrylic acid‐co‐ethylene glycol dimethacrylate) monolith in a defined zone were successfully achieved. Different factors including the surface grafting, polymerization time, PDMS elastic properties (ratio of oligomer/curing reagent) and UV intensity that affect the monolith synthesis in the PDMS microchannel were investigated and optimized. Dopamine, a model analyte, has been online microextracted, eluted, electrophoresized and electrochemically detected in the microchip, with a mean concentration enrichment factor of 80 ( n =3). The results demonstrated that the PPM could be synthesized successfully in the PDMS microchip with a homogeneous structure and excellent mechanical properties. Furthermore, owing to the intrinsic character using PDMS in large‐scale integrated microsystems, the implantation of PPM pretreatment units in PDMS microchips would make it possible to deal with complicated analytical processes in a high‐throughput way.