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“Click” chemistry‐based surface modification of poly(dimethylsiloxane) for protein separation in a microfluidic chip
Author(s) -
Zhang Zhaowei,
Feng Xiaojun,
Xu Fei,
Liu Xin,
Liu BiFeng
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000208
Subject(s) - microfluidics , click chemistry , microfluidic chip , surface modification , nanotechnology , chip , chemistry , separation method , lab on a chip , chromatography , materials science , polymer chemistry , computer science , telecommunications
Abstract “Click” chemistry‐based surface modification strategy was developed for PDMS microchips to enhance separation performance for both amino acids and proteins. Alkyne‐PEG was synthesized by a conventional procedure and then “click” grafted to azido‐PDMS. FTIR absorption by attenuated total reflection and contact angle measurements proved efficient grafting of alkyne‐PEG onto PDMS surface. Manifest EOF regulation and stability of PEG‐functionalized PDMS microchips were illustrated via EOF measurements and protein adsorption investigations. The stability of nonspecific protein adsorption resistance property was investigated up to 30 days. Separation of fluorescence‐labeled amino acids and proteins was further demonstrated with high repeatability and reproducibility. Comparison of protein separation using PDMS microchips before and after surface modification suggested greatly improved electrophoretic performance of the PEG‐functionalized PDMS microchips. We expect the “click” chemistry‐based surface modification method to have wide applications in microseparation of proteins with long‐term surface stability.