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A miniaturized spatial temperature gradient capillary electrophoresis system with radiative heating and automated sample introduction for DNA mutation detection
Author(s) -
Xu ZhangRun,
Li Qi,
Fan XiaoFeng,
Zhang HuiDan,
Fang Jin
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000205
Subject(s) - temperature gradient , analytical chemistry (journal) , capillary action , capillary electrophoresis , materials science , graphite , temperature control , chromatography , electrokinetic phenomena , sample (material) , chemistry , nanotechnology , physics , quantum mechanics , composite material , thermodynamics
A miniaturized spatial temperature gradient CE system with automated sample introduction for DNA mutation detection was established. Continuous electrokinetic sample injection was achieved by combining an automated slotted‐vial array sample introduction device to the spatial temperature gradient CE system. The temperature gradient was produced by a radiative heating system with a single graphite block heater, and the stability of the temperature gradient was investigated. The temperature variation of each measure point was 0.12–0.21% RSD ( n =7) within 6 h. A 14‐cm Teflon AF‐coated silica capillary was used both as the separation channel and as the liquid‐core waveguide tube of fluorescence signal. Under a temperature gradient from 54.8 to 59.5°C, a low range control mutation standard (209 bp) was separated within 4 min with only 5.6 nL sample consumption. Automated continuous sample introducing and changing were realized with a carryover of 3.3%. Utility of the system was further demonstrated by detecting K‐ras gene mutations in paraffin tissue sections from two colorectal cancer patients.