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Portable capillary electrophoresis system for identification of cattle breeds based on DNA mobility
Author(s) -
Lee Miji,
Cho Keunchang,
Yoon Duhak,
Yoo Dong Jin,
Kang Seong Ho
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000199
Subject(s) - capillary electrophoresis , microsatellite , snp , analytical chemistry (journal) , dna , capillary action , chromatography , materials science , biology , chemistry , gene , genetics , single nucleotide polymorphism , allele , genotype , composite material
A portable CE system was developed for the identification of cattle breeds. The system had a width of 44 cm, depth of 27 cm, height of 13 cm, and a weight of only ∼8 kg and included an LIF detector, with everything integrated into a small box. The specific sizes of genes were quickly separated and detected with a high sensitivity based on the difference in the DNA mobility using a diode‐pumped solid‐state LIF detector. Using this system, the 100‐bp DNA ladder was analyzed under a 1.0% PVP ( M r =300 000) sieving gel matrix in a fused silica capillary with LODs of 4.4–13.0 pg/μL ( S / N =3) for 100–3000 bp DNAs, which indicates ten times improved value than other commercialized portable CE system. The migration times and the peak areas showed good reproducibilities with relative standard deviations that were less than 0.49 and 1.3% ( n =5), respectively. Based on the difference in the DNA mobility of the microsatellite and SNP markers, Korean cattle and Holstein were exactly identified as the model cattle breeds within 32 and 3.5 min, respectively.