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Assay of vitamin B in urine by capillary electrochromatography with methacrylate‐based monolithic column
Author(s) -
Wei Xiaoyi,
Qi Li,
Qiao Juan,
Yao Chunhe,
Wang Fuyi,
Chen Yi
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000147
Subject(s) - chromatography , analyte , capillary electrochromatography , chemistry , monolithic hplc column , riboflavin , detection limit , calibration curve , methacrylate , capillary action , resolution (logic) , urine , capillary electrophoresis , high performance liquid chromatography , materials science , copolymer , biochemistry , composite material , polymer , organic chemistry , artificial intelligence , computer science
A novel and simple method for the separation of major vitamin B analytes, such as thiamine, riboflavin, nicotinamide, vitamin B4, pyridoxine, has been developed by CEC using the monolithic column. It has been found that the baseline separation of the five analytes could be achieved with 5.0 mM phosphate buffer at pH 4.0. Compared with the open‐tubular capillary and the bared capillary columns, the poly(butylmethacrylate‐co‐ethylene glycol dimethacrylate) monolithic capillary could exhibit the best resolution in the analysis. Then the method was validated and the linear calibration ranges were obtained with correlation coefficients more than 0.997. The precision and the recovery were also investigated and showed a good result. Furthermore, the proposed method was successfully applied to assay the concentration of vitamin B analytes and the metabolic situation in human urine samples.