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On‐line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV‐absorption detection
Author(s) -
Zeisbergerová Marta,
Řemínek Roman,
Mádr Aleš,
Glatz Zdeněk,
Hoogmartens Jos,
Van Schepdael Ann
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000145
Subject(s) - capillary electrophoresis , chromatography , chemistry , dextromethorphan , capillary action , polyacrylamide , micellar electrokinetic chromatography , sodium , materials science , medicine , organic chemistry , anesthesia , polymer chemistry , composite material
This study presents the in‐capillary enzymatic biotransformation of dextromethorphan, an antitusive drug and opioid receptor antagonist, and subsequent electrophoretic separation of its products. The study includes the optimization of separation parameters to fulfill the requirements of an online microreaction. The analyses were performed in a bare fused‐silica capillary using 100 mM sodium tetraborate (pH 10.0) mixed with linear polyacrylamide (20%, v/v) and 2‐propanol (10%, v/v). This BGE was suitable for monitoring both off‐line and in‐capillary incubations. The partial filling technique enabled the enzymatic reaction to be carried out in its optimal environment (20 mM sodium phosphate, pH 7.4). Finally, in‐capillary microreaction in the presence of cytochrome P450 3A4 gave satisfactory outcomes.