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Ultra‐fast adenosine 5′‐triphosphate, adenosine 5′‐diphosphate and adenosine 5′‐monophosphate detection by pressure‐assisted capillary electrophoresis UV detection
Author(s) -
Zinellu Angelo,
Sotgia Salvatore,
Scanu Bastianina,
Pisanu Elisabetta,
Sanna Manuela,
Franca Usai Maria,
Deiana Luca,
Carru Ciriaco
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000138
Subject(s) - adenosine , adenosine triphosphate , adenosine diphosphate , chemistry , capillary electrophoresis , chromatography , adenosine monophosphate , nucleotide , adenine nucleotide , electrolyte , sodium , electrophoresis , analyte , biochemistry , electrode , biology , platelet , platelet aggregation , organic chemistry , gene , immunology
Herein, we report a new CE method to measure adenine nucleotides adenosine 5′‐triphosphate, adenosine 5′‐diphosphate, and adenosine 5′‐monophosphate in red blood cells. For this purpose, 20 mmol/L sodium acetate buffer at pH 3.80 was used as running electrolyte, and the separation was performed by the simultaneous application of a CE voltage of 25 kV and an overimposed pressure of 0.2 psi from inlet to outlet. A rapid separation of these analytes in less than 1.5 min was obtained with a good reproducibility for intra‐ and inter‐assay (CV<4 and 8%, respectively) and an excellent analytical recovery (from 98.3 to 99%). The applicability of our method was proved by measuring adenine nucleotides in red blood cells.