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Development of neurons on micropatterns reveals that growth cone responds to a sharp change of concentration of laminin
Author(s) -
Xing Shige,
Liu Wenwen,
Huang Zhuo,
Chen Long,
Sun Kang,
Han Dong,
Zhang Wei,
Jiang Xingyu
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000133
Subject(s) - growth cone , neurite , laminin , biophysics , actin , hippocampal formation , chemistry , axon , neuroscience , materials science , nanotechnology , microbiology and biotechnology , biology , biochemistry , in vitro , cell
In this report we fabricated laminin (LN) stripes on a background of poly‐ L ‐lysine as substrates for the growth of rat hippocampal neurons, and found that a sharp change of the concentration of LN guides the growth of neurites by leading the growth cones in a time‐ and space‐dependent manner. The percentage of neurites that grow along the edge of LN stripes (where there is a sharp change of concentration) decreases as a function of the concentration of LN under a threshold value. The actin cytoskeleton plays an important role in the process of growth cone's response to the sharp change of concentration of LN on micropatterns. We believe that the findings here are useful for not only fundamental studies in neuroscience, but also helpful for the design of devices or chips for nervous prosthesis.