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Lab‐on‐a‐chip device for continuous particle and cell separation based on electrical properties via alternating current dielectrophoresis
Author(s) -
Çetin Barbaros,
Li Dongqing
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000107
Subject(s) - dielectrophoresis , joule heating , electric field , materials science , microfluidics , electrode , electrophoresis , particle (ecology) , current (fluid) , voltage , alternating current , optoelectronics , electric current , nanotechnology , composite material , electrical engineering , chemistry , chromatography , physics , oceanography , engineering , quantum mechanics , geology
A novel alternating current‐dielectrophoresis microfluidic chip was developed to separate particles and cells continuously by their electric properties. The flow is induced by pressure gradient. A pair of simple, 3‐D electrodes was used to achieve a localized nonuniform electric field. Dielectrophoretic force is generated in the transverse direction to the flow by inserting the electrodes along the channel side walls. The localized electric field is important to reduce the Joule heating and any adverse effects of electrical field on biological cells. Latex particles of different sizes and white blood cells (8–12 μm) were manipulated successfully and the separation of 10 μm latex particles and white blood cells based on their different electrical properties was demonstrated.