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Detection of immunogenic parasite and host‐specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii
Author(s) -
Yeng Chen,
Osman Emelia,
Mohamed Zeehaida,
Noordin Rahmah
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000038
Subject(s) - toxoplasma gondii , biology , toxoplasmosis , glycoprotein , western blot , blot , parasite hosting , antigen , monoclonal antibody , microbiology and biotechnology , antibody , virology , immunology , gene , biochemistry , world wide web , computer science
Toxoplasma gondii infection in pregnant women may result in abortion and foetal abnormalities, and may be life‐threatening in immunocompromised hosts. To identify the potential infection markers of this disease, 2‐DE and Western blot methods were employed to study the parasite circulating antigens and host‐specific proteins in the sera of T. gondii ‐infected individuals. The comparisons were made between serum protein profiles of infected ( n =31) and normal ( n =10) subjects. Antigenic proteins were identified by immunoblotting using pooled sera and monoclonal anti‐human IgM‐HRP. Selected protein spots were characterised using mass spectrometry. Prominent differences were observed when serum samples of T. gondii ‐infected individuals and normal controls were compared. A significant up‐regulation of host‐specific proteins, α 2 ‐HS glycoprotein and α 1 ‐B glycoprotein, was also observed in the silver‐stained gels of both active and chronic infections. However, only α 2 ‐HS glycoprotein and α 1 ‐B glycoprotein in the active infection showed immunoreactivity in Western blots. In addition, three spots of T. gondii proteins were detected, namely (i) hypothetical protein chrXII: 3984434‐3 TGME 49, (ii) dual specificity protein phosphatase, catalytic domain TGME 49 and (iii) NADPH‐cytochrome p450 reductase TGME 49. Thus, 2‐DE approach followed by Western blotting has enabled the identification of five potential infection markers for the diagnosis of toxoplasmosis: three are parasite‐specific proteins and two are host‐specific proteins.