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Integration of on‐chip peristaltic pumps and injection valves with microchip electrophoresis and electrochemical detection
Author(s) -
Bowen Amanda L.,
Martin R. Scott
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201000029
Subject(s) - peristaltic pump , microfluidics , amperometry , capillary electrophoresis , chromatography , peristalsis , analyte , chip , electrophoresis , materials science , chemistry , nanotechnology , electrochemistry , computer science , electrode , engineering , mechanical engineering , telecommunications , biochemistry
A microfluidic approach that integrates peristaltic pumping from an on‐chip reservoir with injection valves, microchip electrophoresis and electrochemical detection is described. Fabrication and operation of both the peristaltic pumps and injection valves were optimized to ensure efficient pumping and discrete injections. The final device uses the peristaltic pumps to continuously direct sample from a reservoir containing a mixture of analytes to injection valves that are coupled with microchip electrophoresis and amperometric detection. The separation and direct detection of dopamine and norepinephrine were possible with this approach and the utility of the device was demonstrated by monitoring the stimulated release of these neurotransmitters from a layer of cells introduced into the microchip. It is also shown that this pumping/reservoir approach can be expanded to multiple reservoirs and pumps, where one reservoir can be addressed individually or multiple reservoirs sampled simultaneously.