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Comparison of serum versus plasma collection in gas chromatography – Mass spectrometry‐based metabolomics
Author(s) -
Dettmer Katja,
Almstetter Martin F.,
Appel Inka J.,
Nürnberger Nadine,
Schlamberger Gregor,
Gronwald Wolfram,
Meyer Heinrich H. D.,
Oefner Peter J.
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900778
Subject(s) - chemistry , chromatography , metabolomics , metabolite , mass spectrometry , gas chromatography–mass spectrometry , gas chromatography , biochemistry
Bovine serum, EDTA‐plasma and EDTA‐plasma fortified with acetylsalicylic acid (ASA) as antioxidant were compared with regard to their suitability for metabolomic studies. Metabolic fingerprints were generated from GC‐TOF‐MS data using the Leco ChromaTOF ® software in combination with the in‐house retention time correction and data alignment tool INCA. A total of 6, 9 and 21 significant features with a false discovery rate of <0.05 were identified by INCA upon comparing EDTA‐ versus EDTA‐ASA‐plasma, EDTA‐plasma versus serum and EDTA‐ASA‐plasma versus serum, respectively. To confirm that the observed signal intensities in the GC‐TOF‐MS fingerprints reflected true metabolite abundances, 19 amino acids, glucose and 6 organic acids were quantified by means of GC‐MS using stable‐isotope‐labeled internal standards. As observed with the fingerprints, only the concentrations of lactate and citrate were found to be significantly lower in EDTA‐plasma and serum, respectively, whereas the concentrations of the other metabolites were similar among the three sample types investigated.