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A strong inorganic acid‐initiated methacrylate polymerization strategy for room temperature preparation of monolithic columns for capillary electrochromatography
Author(s) -
Wang ManMan,
Wang HeFang,
Jiang DongQing,
Wang ShanWei,
Yan XiuPing
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900756
Subject(s) - capillary electrochromatography , polymerization , methacrylate , monolith , monolithic hplc column , chemical engineering , naphthalene , thiourea , alkyl , polymer , materials science , chemistry , chromatography , organic chemistry , catalysis , high performance liquid chromatography , capillary electrophoresis , engineering
A facile strong inorganic acid‐initiated methacrylate polymerization strategy was developed for fabricating monolithic columns at room temperature. The prepared monoliths were characterized by FTIR spectrometry, mercury intrusion porosimeter and SEM, while their performance was evaluated by CEC for the separation of various types of compounds including alkyl benzenes, polycyclic aromatic hydrocarbons, nonsteroidal anti‐inflammatory drugs, anilines, and nitrophenol isomers. The column‐to‐column and batch‐to‐batch reproducibility for the prepared monoliths in terms of the RSD of EOF flow velocity, retention factor, and the minimum plate height of naphthalene ranged from 3.4 to 12.4%. The fabricated monoliths gave excellent performance for the separation of the test neutral compounds with the theoretical plates of 170 000–232 000 plates per meter for thiourea, and 77 400–112 300 plates per meter for naphthalene. The proposed strong inorganic acid‐initiated methacrylate polymerization strategy is a promising alternative for fabricating organic polymer‐based monoliths.