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Study of electromigration effects on a pH boundary during the on‐line electrokinetic preconcentration by capillary electrophoresis
Author(s) -
Vítková Kateřina,
Petr Jan,
Maier Vítězslav,
Znaleziona Joanna,
Ševčík Juraj
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900663
Subject(s) - electrokinetic phenomena , electrolyte , isotachophoresis , chemistry , capillary electrophoresis , chromatography , electrophoresis , micellar electrokinetic chromatography , analyte , dissociation (chemistry) , conductivity , sodium , solvent , electromigration , methanol , analytical chemistry (journal) , materials science , organic chemistry , electrode , composite material
A contribution to the description of electrokinetic effects on the pH boundary formed by sodium borate pH 9.5 and sodium phosphate pH 2.5 electrolytes for on‐line preconcentration of weak acids is presented in this article. Simulations of electrokinetic injections together with experimental studies using contactless conductivity detection verified that the preconcentration is induced mainly by dissociation changes of analytes on the pH boundary and transient ITP state. Moreover, a study of the addition of organic solvent to the injection electrolyte was performed with impressive results. Subnanomolar LODs of hydroxybenzoic acids were achieved with 80% of methanol in the injection electrolyte which represents more than 70 000‐fold preconcentration in comparison with classical CZE method.