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Investigations on the migration behavior of insulin and related synthetic analogues in CZE, MEKC and MEEKC employing different surfactants
Author(s) -
Haunschmidt Manuela,
Ortner Karin,
Hainz Katharina,
Bradt Elke,
Sternbauer Lukas,
Buchberger Wolfgang,
Klampfl Christian W.
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900547
Subject(s) - chemistry , micelle , micellar electrokinetic chromatography , chromatography , insulin , selectivity , capillary electrophoresis , biochemistry , biology , organic chemistry , aqueous solution , endocrinology , catalysis
The retention/migration behavior of insulin and five synthetic insulin analogues in CZE, MEKC and MEEKC employing seven different detergents within the latter two techniques has been investigated. Substantial changes in separation selectivity in MEKC could be observed for several insulins when moving from SDS to cholate‐based micellar systems. Customized separations could be achieved by using mixtures of SDS and deoxycholate. A similar effect could be observed in MEEKC although the overall quality of MEEKC separations was inferior to those obtained with MEKC.