Premium
SDS‐PAGE and two‐dimensional maps in a radial gel format
Author(s) -
Millioni Renato,
Miuzzo Manuela,
Antonioli Paolo,
Sbrignadello Stefano,
Iori Elisabetta,
Dosselli Ryan,
Puricelli Lucia,
Kolbe Markus,
Tessari Paolo,
Righetti Pier Giorgio
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900526
Subject(s) - isoelectric focusing , resolution (logic) , dimension (graph theory) , square (algebra) , chromatography , surface (topology) , chemistry , analytical chemistry (journal) , computer science , mathematics , geometry , combinatorics , artificial intelligence , biochemistry , enzyme
A novel method for performing 2‐D map analysis is here reported, consisting in a modification of the second dimension run, which is performed not in a conventional square‐ or rectangular‐size gel, but in a radial surface. This has the advantage of permitting resolution of closely adjacent bands, representing strings of isoforms of similar or identical mass but of closely spaced isoelectric points. When used in a mono‐dimensional, SDS‐PAGE format, this system allows the simultaneous running of 62 sample tracks. Examples are given of separation of plasma and urinary proteins.