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Comparison and prediction of the retention in micellar electrokinetic chromatography and microemulsion electrokinetic chromatography for disubstituted benzenes
Author(s) -
Angkanasiriporn Siriporn,
Singsung Wuttipong,
Petsom Amorn,
Nhujak Thumnoon
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900486
Subject(s) - alkylbenzenes , micellar electrokinetic chromatography , chemistry , electrokinetic phenomena , microemulsion , kovats retention index , chromatography , pulmonary surfactant , analytical chemistry (journal) , benzene , organic chemistry , gas chromatography , electrophoresis , biochemistry
Abstract Retention index ( I ), rather than retention factor ( k ), was found to be a more reasonable parameter for comparison of the relative affinity of disubstituted benzenes in MEEKC and MEKC, due to independent of I with the SDS surfactant concentration. MEKC and MEEKC may give similar or different I values, depending on types of moieties. With known I and K ow for alkylbenzenes as references in MEKC and MEEKC, the values of K ow for disubstituted benzenes can be estimated from the observed I values, where K ow is the octanol–water distribution constant. In addition, a group additive approach can be used to predict I for disubstituted benzenes with different moieties from the average observed I for the disubstituted benzenes with same moieties. However, electronic effects and/or intramolecular interaction may result in the different observed I from prediction.