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Capillary modified with covalently attached coating for enhanced CE separation of biopolymers
Author(s) -
He Yuan,
Wei Yinmao,
Zheng Xiaohui,
Zheng Jianbin
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900463
Subject(s) - covalent bond , coating , capillary action , chemistry , capillary electrophoresis , capillary electrochromatography , chromatography , biopolymer , chemical engineering , materials science , nanotechnology , organic chemistry , polymer , composite material , engineering
δ‐Gluconolactone was covalently coupled with aminopropyl‐derivatized capillary, creating hydrophilic brushes on the inner wall of the capillary. The hydrophilic coating provided suppression of EOF and minimized protein adsorption, resulting in the separation of basic proteins and DNA with efficiencies up to 450 000 plates/m. The intra‐ and inter‐day repeatabilities of the coating referring to the migration times of the four tested proteins were satisfactory with RSD of no more than 1.1 and 1.8% ( n =5), respectively. Two hundred consecutive runs were performed with negligible change in migration times and efficiency.