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Monolithic silica capillary columns with immobilized cellulose tris(3,5‐dimethylphenylcarbamate) for enantiomer separations in CEC
Author(s) -
He Chiyang,
Hendrickx Ans,
Mangelings Debby,
SmeyersVerbeke Johanna,
Vander Heyden Yvan
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900456
Subject(s) - monolith , cellulose , enantiomer , monolithic hplc column , chemistry , chromatography , tris , silica gel , capillary electrochromatography , capillary action , polymerization , copolymer , condensation polymer , capillary electrophoresis , polymer chemistry , high performance liquid chromatography , polymer , materials science , organic chemistry , catalysis , biochemistry , composite material
Two types of monolithic silica capillary columns with an immobilized cellulose tris (3,5‐dimethylphenylcarbamate) (CDMPC) selector were prepared for enantiomer separations in CEC. The monolithic columns were prepared by a sol‐gel process in fused‐silica capillaries. CDMPC was then either immobilized on a silica monolith through an intermolecular polycondensation of the cellulose derivative containing a triethoxysilyl group, or on a vinylized silica monolith through radical copolymerization of the cellulose derivative, which also contained a vinyl group. IR spectra confirmed the successful immobilization of CDMPC on both columns. Eleven chiral compounds were used to evaluate the enantioselectivity on both column types. Results indicated that the columns obtained via polycondensation had higher separation ability than those obtained via radical polymerization, and that they showed satisfactory run‐to‐run repeatability and stability. These new techniques thus provide strategies for preparing immobilized polysaccharide‐based chiral silica monolithic capillary columns for chiral separations by means of CEC.