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CE‐MALDI interface based on inkjet technology
Author(s) -
Vannatta Michael W.,
Whitmore Colin D.,
Dovichi Norman J.
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900414
Subject(s) - chromatography , capillary electrophoresis , nozzle , capillary action , matrix (chemical analysis) , materials science , analytical chemistry (journal) , electrophoresis , chemistry , composite material , physics , thermodynamics
An ink jet printer valve and a nozzle were used to deliver matrix and sample from an electrophoresis capillary onto a MALDI plate. The system was evaluated by the separation of a set of standard peptides. That separation generated up to 40 000 theoretical plates in less than 3 min. Detection limits were 500 amol for an ABI TOF‐TOF instrument and 2 fmol for an ABI Q‐TOF instrument. Over 70% coverage was obtained for the tryptic digest of α‐lactalbumin in less than 2.5 min.