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A new evaluation technique for the detection of impurities in purified proteins via CE with native UV‐LIF
Author(s) -
Rodat Audrey,
Couderc Bettina,
Feurer Bernard,
Couderc François
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900369
Subject(s) - impurity , chemistry , detector , sensitivity (control systems) , analytical chemistry (journal) , absorption (acoustics) , materials science , chromatography , optics , physics , organic chemistry , electronic engineering , engineering , composite material
An analytical methodology for quality control analyses of IgG and their impurities is presented using a new UV‐LIF (266 nm) detector inside the cassette of a CE instrument and its performance was evaluated. The observed sensitivity was very close to that obtained by silver staining of slab gels (LOD of 25 ng/mL), while the sensitivity of the analysis is 80 times better than with CE/UV absorption (214 nm). Examples of the analysis of pharmaceutical and other commercial IgGs are provided and the kinetics of the reduction of IgG by β‐mercaptoethanol is reported, demonstrating the ease of performing the analysis.