Premium
Temperature gradient focusing in miniaturized free‐flow electrophoresis devices
Author(s) -
Becker Marco,
Mansouri Abraham,
Beilein Cornelia,
Janasek Dirk
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900359
Subject(s) - temperature gradient , isoelectric focusing , free flow electrophoresis , chromatography , electrophoresis , balanced flow , analyte , isoelectric point , flow (mathematics) , fluorescence , chemistry , concentration gradient , analytical chemistry (journal) , materials science , gel electrophoresis of proteins , mechanics , polyacrylamide gel electrophoresis , physics , optics , biochemistry , quantum mechanics , meteorology , enzyme
Temperature gradient focusing is a method to separate and focus any charged analytes even without accessible isoelectric point, and has been already widely used in CE. In this paper, we demonstrate the application of temperature gradient focusing to free‐flow electrophoresis. Besides focusing and separation experiments of proteins, the stability of the temperature gradient under flow conditions and the temperature dependence of fluorescence dyes have also been investigated.